Those responses work for WebI but not for Crystal…
Crystal includes DistinctCount as a summary type - just use that.
Is Column B the number of samples or the sample ID? If it’s the sample ID, you’ll need to group on the date field to get this to work. You can either set the group to show by the month instead of showing all of the dates and create a distinct count summary by using the summary button and setting the distinct count for the date group or you can create a formula that will look something like this: